€ 25.99 · 4.8 (502) · In Magazzino
Melanoma inhibitory activity/cartilage-derived retinoicacid-sensitive protein (MIA/CD-RAP) is a protein expressed and secreted by chondrocytes and cartilaginous tissues. MIA/CD-RAP-deficient mice develop milder osteoarthritis than wildtype mice. In this study, we investigated MIA/CD-RAP downstream targets to explain this reduced disease development. As a possible mediator, we could detect matrix metalloproteinase 13 (MMP13), and the influence of MIA/CD-RAP on MMP13 regulation was analyzed in vitro using SW1353 chondrosarcoma cells and primary chondrocytes. The femoral head cartilage of WT and MIA/CD-RAP −/− mice were cultured ex vivo to further investigate MMP13 activity. Finally, osteoarthritis was surgically induced via DMM in C57BL/6 mice, and the animals were treated with an MIA/CD-RAP inhibitory peptide by subcutaneously implanted pellets. MMP13 was regulated by MIA/CD-RAP in SW1353 cells, and MIA/CD-RAP −/− murine chondrocytes showed less expression of MMP13. Further, IL-1β-treated MIA/CD-RAP −/− chondrocytes displayed less MMP13 expression and activity. Additionally, MIA/CD-RAP-deficient ex vivo cultured cartilage explants showed less MMP13 activity as well as reduced cartilage degradation. The mice treated with the MIA/CD-RAP inhibitory peptide showed less osteoarthritis development. Our findings revealed MIA/CD-RAP as a new regulator of MMP13 and highlighted its role as a potential new target for osteoarthritis therapy.
IJMS, Free Full-Text
MitoQuicLy: A high-throughput method for quantifying cell-free DNA from human plasma, serum, and saliva - ScienceDirect
Effect of calendar ageing on the cycle life of anode-free full-cells.
Cells, Free Full-Text, gordon hayward sofascore
Cells, Free Full-Text, gordon hayward sofascore
Cell-free methods to produce structurally intact mammalian membrane proteins
Cells, Free Full-Text, gordon hayward sofascore
Remote immune processes revealed by immune-derived circulating cell-free DNA
Cell-free prediction of protein expression costs for growing cells
Cells, Free Full-Text, freecell online 100
An illustration of the full-duplex cell-free massive MIMO system.
Cell-free biomanufacturing - ScienceDirect
Cell-to-cell infection by HIV contributes over half of virus infection
Cell-free gene expression: an expanded repertoire of applications
Medical Alert Wallet Card Template Awesome Viruses Free Full Text Vero Cells as A Mammalian Cell